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All samplings were taken from 0900 to 2100 of the day after next.Microscopic MethodGlycerol (120 ml) (Assay (GC), 99.0%; Wako Pure Chemical Industries, Ltd, Osaka, Japan), 20 g gelatin (Wako Pure Chemical Industries, Ltd, Osaka, Japan), 1.6 mg gentian violet R (Wako Pure Chemical Industries, Ltd, Osaka, Japan) dissolved in 4 ml ethanol (99.5%; Wako Pure Chemical Industries, Ltd, Osaka, Japan), 1 ml phenol standard solution (1 mg ml1 water solution), 70 ml ultrapure water (MilliQ Gradient A10 System; Mil